| District name | City | County name |
|---|---|---|
| Ahfachkee Day School | Clewiston | Hendry County |
| Alachua | Gainesville | Alachua County |
| Baker | Macclenny | Baker County |
| Bay | Panama City | Bay County |
| Bradford | Starke | Bradford County |
| Brevard Public Schools | VIERA | Brevard County |
| Broward | Fort Lauderdale | Broward County |
| Calhoun | Blountstown | Calhoun County |
| Charlotte | Port Charlotte | Charlotte County |
| Citrus | Inverness | Citrus County |
| Clay | Green Cove Springs | Clay County |
| Collier | Naples | Collier County |
| Columbia | Lake City | Columbia County |
| Deaf or Blind | St. Augustine | St. Johns County |
| DeSoto County Schools | Arcadia | DeSoto County |
| Dixie | Cross City | Dixie County |
| Duval County Public Schools | Jacksonville | Duval County |
| Escambia | Pensacola | Escambia County |
| Famu Lab Sch | Tallahassee | Leon County |
| Fau Lab Sch | Boca Raton | Palm Beach County |
| Fl Virtual | Orlando | Orange County |
| Flagler | Bunnell | Flagler County |
| Franklin | Eastpoint | Franklin County |
| Fsu Lab Sch | Tallahassee | Leon County |
| Gadsden | Quincy | Gadsden County |
| Gilchrist | Trenton | Gilchrist County |
| Glades | Moore Haven | Glades County |
| Gulf | Port St. Joe | Gulf County |
| Hamilton | Jasper | Hamilton County |
| Hardee | Wauchula | Hardee County |
| Hendry | LaBelle | Hendry County |
| Hernando | Brooksville | Hernando County |
| Highlands | Sebring | Highlands County |
| Hillsborough | Tampa | Hillsborough County |
| Holmes | Bonifay | Holmes County |
| Indian River | Vero Beach | Indian River County |
| Jackson | Marianna | Jackson County |
| Jefferson | Monticello | Jefferson County |
| Lafayette | Mayo | Lafayette County |
| Lake | Tavares | Lake County |
| Lee | Fort Myers | Lee County |
| Leon | Tallahassee | Leon County |
| Levy | Bronson | Levy County |
| Liberty | Bristol | Liberty County |
| Madison | Madison | Madison County |
| Manatee | Bradenton | Manatee County |
| Marion | Ocala | Marion County |
| Martin | Stuart | Martin County |
| Miami-Dade County Public Schools | Miami | Miami-Dade County |
| Miccosukee Indian School | Miami | Miami-Dade County |
| Monroe | Key West | Monroe County |
| Nassau | Fernandina Beach | Nassau County |
| Okaloosa | Fort Walton Beach | Okaloosa County |
| Okeechobee | Okeechobee | Okeechobee County |
| Orange County Public Schools | Orlando | Orange County |
| Osceola | Kissimmee | Osceola County |
| Palm Beach | West Palm Beach | Palm Beach County |
| Pasco | Land O' Lakes | Pasco County |
| Pinellas | Largo | Pinellas County |
| Polk | Bartow | Polk County |
| Putnam | Palatka | Putnam County |
| Santa Rosa | Milton | Santa Rosa County |
| Sarasota | Sarasota | Sarasota County |
| Seminole | Sanford | Seminole County |
| St. Johns | St. Augustine | St. Johns County |
| St. Lucie | Port St. Lucie | St. Lucie County |
| Sumter | Bushnell | Sumter County |
| Suwannee | Live Oak | Suwannee County |
| Taylor | Perry | Taylor County |
| Uf Lab Sch | Gainesville | Alachua County |
| Union | Lake Butler | Union County |
| Volusia | DeLand | Volusia County |
| Wakulla | Crawfordville | Wakulla County |
| Walton | DeFuniak Springs | Walton County |
| Washington | Chipley | Washington County |